C57BL/6小鼠急性溃疡性结肠炎动物模型的对比及优化
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摘要：
[目的]对3种常用方法(DSS、TNBS、OXZ)诱导的 C57BL/6 小鼠急性溃疡性结肠炎(UC)动物模型进行对比研究及方法优化。[方法]选取一般状态,体质量变化率、DAI评分、结肠长度、结肠组织病理学损伤和炎症因子几个方面来评价造模效果，[结果]3种模型组小鼠均出现不同程度的体质量下降，稀便以及便血等急性溃疡性结肠炎临床症状，与正常组比，各模型组小鼠 DAI评分显著升高(P<0.01)，结肠长度明显缩短(P<0.05)。组织病理学检查可见模型组小鼠结肠出现溃疡、炎性细胞浸润等病理特征。[结论]3种造模方法均可以成功建立 C57BL/6 小鼠急性实验性溃疡性结肠炎模型，但不同模型组小鼠在症状、发病时间、恢复时间、病理和免疫结果方面存在一定差异。
[Objective]To compare and optimize three commonly used methods( DSS, TNBS, OXZ) for inducing acute ulcerative colitis(UC) animal models in C57BL/6 mice. [Methods]Gieneral condition, weight change rate, DAl score, colonlength, colonic histopathological damage and inlammatory factors were selected to evaluate the modeling effect, [Results] Mice in all three model groups exhibited varying degrees of weight loss, diarrhea, and rectal bleeding, typical clinical symptoms of acute UC, Compared with the control group, mice in each model group showed significantly elevated DAl scores(P<0.01) and noticeably shortened colon lengths(P<0.05). Histopathological examination revealed ulceration and inflammatory cell infiltration in the colons of model group mice, [Conclusion] All three modeling methods successfully established acute experimental ulcerative colitis models in C57BL/6 mice,but there were certain differences among the model groups in terms of symptoms, onset time, recovery time, athology and the immunological results.
作者：
李萌，马富智，唐泽惟，章从恩，马致洁，饶全
Li Meng，Ma Fuzhi，Tang Zewei，Zhang Congen，Ma Zhijie，Rao Quan
机构地区：
首都医科大学附属北京友谊医院；云南中医药大学中药学院；首都医科大学附属北京地坛医院药学部
引用本文：
李萌，马富智，唐泽惟等。C57BL/6小鼠急性溃疡性结肠炎动物模型的对比及优化 [ ] ].河南师范大学学报(自然科学版),2025,53(4):124-131.(li Meng, Ma Fuzhi, Tang Zewei,et al.Comparison and optimization of acuteulcerative colitis animal models induced in C57BL/6 mice[ J ].Joumal of Henan Normal University( Natural Science Edition),2025,53(4):124-131.D01:10,16366/j.cnki.1000-2367.2024.03.27.0002.)
基金：
国家自然科学基金；北京市自然科学基金
关键词：
炎症性肠病；溃疡性结肠炎；动物模型；C57BL/6小鼠
inflammatory bowel disease; ulcerative colitis; animal model; C57BL/6 mice
分类号：
R574
