南唐钦陵墓室腐蚀壁画丝状蓝藻的形态分析与分子鉴定
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摘要：
从南京市南唐钦陵墓室腐蚀壁画采集的样本中分离了一株丝状蓝藻，该藻株与细点丝藻目(Ocula-tellales)细点丝藻科(Oculatelaceae)阿尔伯塔(Alberanig)属的藻种具有相似的生境。通过形态观察和分子系统发育分析，结果表明：（1）该藻株藻丝体直，或弯曲盘绕，无明显收缢，具鞘鞘无色，紧贴着丝状体，细胞长短于宽，不产生异形胞和厚壁孢子。胞内具8~14条的侧生类囊体，与阿尔伯塔(Albertania)属具有相似的形态特征：（2）通过16S rDNA序列的分析，发现该藻株与阿尔伯塔(Albertania)属相似性达 98.06%，在进化上聚为一支;这是国内首次对幕穴特殊生境中的蓝藻进行分离，并采用形态和分子系统发育并结合生态学特征分析，鉴定的蓝藻新记录属，对于文物保护研究及丰富蓝藻的多样性具有重要意义。
A filamentous cyanobacteria strain was isolated from deteriorated wall paintings of Qinling Tomb in the Southern Tang Dynasty, The habitat of the strain was similar with Albertania(Oculatellales, Oculatellaceae) genus. Through morphological and molecular phylogenetic analysis, the results showed that: (1)The filaments were straight, or curved and coiled, sheathed, without obvious restraint, The sheath was colorless, tightly attached to the trichomes. The cells were shorter than wide, no heterocytes or akinetes, 8-14 lateral thylakoids in the cell, These morphological characteristics were similar to the genus Albertania ; (2)The 16S rDNA sequence of the strain was 98.06% similarity to the genus Albertania and the phylogenetic analyses based on 16S rDNA sequence showed that the strain was clustered with Albertania. The morphological and molecular phylogenetic evidence supported the strain to be a member of Albertania genus, The genus Albertania is the newly recorded genus in China, This was the first time to isolate cyanobacteria from tomb habitats in China, which was of great significance for the protection of cultural relics and the enrichment of the diversity of cyanobacteria.
作者：
郑凌凌，张琪，李天丽，邢伟，蔡芳芳，虞功亮，宋立荣
Zheng Lingling, Zhang Qi, Li Tianli, Xing Wei, Cai Fangfang, Yu Gongliang, Song Lirong
机构地区：
中国科学院水生生物研究所；中国科学院大学生命科学学院；武汉轻工大学动物科学与营养工程学院
引用本文：
郑凌凌，张琪，李天丽等，南唐钦陵墓室腐蚀壁画丝状蓝藻的形态分析与分子鉴定[J].河南师范大学学报(自然科学版),2025,53(4):118-123.(Zheng Lingling,Zhang Qi, Li Tianli,et al.Morphological analysis and molecular identification of filamentous cyanobacteria from deteriorated wall paintings of Qinling Tomb in the Southern Tang Dynasty [J].Journal of Henan Normal University(Natural Science Edition),2025,53(4):118-123.DO1:10,16366/j.cnki1000-2367.2024.05.26.0001.)
基金：
国家自然科学基金；国家重点研发项目
关键词：
壁画；阿尔伯塔；蓝藻；分类；新记录
wall painting; Albertania ; cyanobacteria; taxonomy; new record
分类号：
Q949.2
